Journal: Acta Biochimica et Biophysica Sinica
Article Title: RASGRP1 targeted by H3K27me3 regulates myoblast proliferation and differentiation in mice and pigs
doi: 10.3724/abbs.2024011
Figure Lengend Snippet: RASGRP1 overexpression promotes the proliferation of C2C12 cells (A) The relative expression levels of RASGRP1 and proliferation marker genes after RASGRP1 overexpression. (B) Detection of C2C12 cell viability by CCK-8 assay at 18 h, 24 h and 30 h after RASGRP1 overexpression. (C) The relative protein levels of the Ki67 and CDK2 genes after RASGRP1 overexpression. (D) EdU staining after RASGRP1 overexpression in C2C12 cells. Scale bar: 100 μm. (E) Detection of the number of S-phase cells by flow cytometry after RASGRP1 overexpression. *P<0.05, **P<0.01.
Article Snippet: The antibodies used were as follows: Ki67 (ab16667; 1:1000; Abcam, Cambridge, UK), CDK2 (PA1547; 1:1000; Boster, Pleasanton, USA), MyoG (sc-12732; 1:500; Santa Cruz Biotechnology, Santa Cruz, USA), MyoD (sc-377460; 1:500; Santa Cruz Biotechnology), MyHC (sc-376157; 1:1000; Santa Cruz Biotechnology), β-Tubulin (GB11017; 1:1000; Servicebio, Wuhan, China), goat anti-mouse IgG (A0216; 1:3000; Beyotime) and goat anti-rabbit IgG (A0208; 1:3000; Beyotime).
Techniques: Over Expression, Expressing, Marker, CCK-8 Assay, Staining, Flow Cytometry